joachim messing Search Results


joachim messing  (Addgene inc)
93
Addgene inc joachim messing
Joachim Messing, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/joachim messing/product/Addgene inc
Average 93 stars, based on 1 article reviews
joachim messing - by Bioz Stars, 2026-04
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puc19  (Addgene inc)
96
Addgene inc puc19
A dual-fluorescence eccDNA biosensor system and CRISPR-C for studying eccDNA biogenesis in human cells. ( A ) Model of TRE-ECC biosensor after two double-stranded breaks by CRISPR-C (Cr1 and Cr2), leading to [ EGFP circle ] and Δ EGFP or EGFP inversion. ( B ) Scheme of TRE-ECC biosensor. ( C ) Experimental outline. ( D ) TRE-ECC plasmid assessment by fluorescence microscopy after Cr1+Cr2 using <t>pUC19</t> as control (uncut). ( E , upper part) Outline of stable genomic integration of TRE-ECC in HEK293T. ( F ) Copy-number assessment by Southern blot with EGFP-probe after KpnI digestion. (E, lower part) Outline for Cr1+Cr2 activation of integrated TRE-ECC. ( G ) Representative result from FACS analysis of clone 1 in the absence or presence of tetracycline (Tet). ( H ) Histograms of fluorescence cell percentages of all 15 isolated TRE-ECC clones after Cr1+Cr2 and FACS analysis. (I) PCR and Sanger sequencing validation of genotypes on exonuclease-treated DNA, displayed in A, after Cr1+Cr2 for TRE-ECC clone 1 and clone 4. C, negative CRISPR control; NC, non-template control. ( J ) Southern blot, probed with EGFP on HEK293T purified and digested DNA from untreated (C, KpnI ) and Cr1+Cr2 treated cells. X = XbaI , H = HindIII .
Puc19, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/puc19/product/Addgene inc
Average 96 stars, based on 1 article reviews
puc19 - by Bioz Stars, 2026-04
96/100 stars
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puc18  (Addgene inc)
93
Addgene inc puc18
A dual-fluorescence eccDNA biosensor system and CRISPR-C for studying eccDNA biogenesis in human cells. ( A ) Model of TRE-ECC biosensor after two double-stranded breaks by CRISPR-C (Cr1 and Cr2), leading to [ EGFP circle ] and Δ EGFP or EGFP inversion. ( B ) Scheme of TRE-ECC biosensor. ( C ) Experimental outline. ( D ) TRE-ECC plasmid assessment by fluorescence microscopy after Cr1+Cr2 using <t>pUC19</t> as control (uncut). ( E , upper part) Outline of stable genomic integration of TRE-ECC in HEK293T. ( F ) Copy-number assessment by Southern blot with EGFP-probe after KpnI digestion. (E, lower part) Outline for Cr1+Cr2 activation of integrated TRE-ECC. ( G ) Representative result from FACS analysis of clone 1 in the absence or presence of tetracycline (Tet). ( H ) Histograms of fluorescence cell percentages of all 15 isolated TRE-ECC clones after Cr1+Cr2 and FACS analysis. (I) PCR and Sanger sequencing validation of genotypes on exonuclease-treated DNA, displayed in A, after Cr1+Cr2 for TRE-ECC clone 1 and clone 4. C, negative CRISPR control; NC, non-template control. ( J ) Southern blot, probed with EGFP on HEK293T purified and digested DNA from untreated (C, KpnI ) and Cr1+Cr2 treated cells. X = XbaI , H = HindIII .
Puc18, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/puc18/product/Addgene inc
Average 93 stars, based on 1 article reviews
puc18 - by Bioz Stars, 2026-04
93/100 stars
  Buy from Supplier

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A dual-fluorescence eccDNA biosensor system and CRISPR-C for studying eccDNA biogenesis in human cells. ( A ) Model of TRE-ECC biosensor after two double-stranded breaks by CRISPR-C (Cr1 and Cr2), leading to [ EGFP circle ] and Δ EGFP or EGFP inversion. ( B ) Scheme of TRE-ECC biosensor. ( C ) Experimental outline. ( D ) TRE-ECC plasmid assessment by fluorescence microscopy after Cr1+Cr2 using pUC19 as control (uncut). ( E , upper part) Outline of stable genomic integration of TRE-ECC in HEK293T. ( F ) Copy-number assessment by Southern blot with EGFP-probe after KpnI digestion. (E, lower part) Outline for Cr1+Cr2 activation of integrated TRE-ECC. ( G ) Representative result from FACS analysis of clone 1 in the absence or presence of tetracycline (Tet). ( H ) Histograms of fluorescence cell percentages of all 15 isolated TRE-ECC clones after Cr1+Cr2 and FACS analysis. (I) PCR and Sanger sequencing validation of genotypes on exonuclease-treated DNA, displayed in A, after Cr1+Cr2 for TRE-ECC clone 1 and clone 4. C, negative CRISPR control; NC, non-template control. ( J ) Southern blot, probed with EGFP on HEK293T purified and digested DNA from untreated (C, KpnI ) and Cr1+Cr2 treated cells. X = XbaI , H = HindIII .

Journal: Nucleic Acids Research

Article Title: CRISPR-C: circularization of genes and chromosome by CRISPR in human cells

doi: 10.1093/nar/gky767

Figure Lengend Snippet: A dual-fluorescence eccDNA biosensor system and CRISPR-C for studying eccDNA biogenesis in human cells. ( A ) Model of TRE-ECC biosensor after two double-stranded breaks by CRISPR-C (Cr1 and Cr2), leading to [ EGFP circle ] and Δ EGFP or EGFP inversion. ( B ) Scheme of TRE-ECC biosensor. ( C ) Experimental outline. ( D ) TRE-ECC plasmid assessment by fluorescence microscopy after Cr1+Cr2 using pUC19 as control (uncut). ( E , upper part) Outline of stable genomic integration of TRE-ECC in HEK293T. ( F ) Copy-number assessment by Southern blot with EGFP-probe after KpnI digestion. (E, lower part) Outline for Cr1+Cr2 activation of integrated TRE-ECC. ( G ) Representative result from FACS analysis of clone 1 in the absence or presence of tetracycline (Tet). ( H ) Histograms of fluorescence cell percentages of all 15 isolated TRE-ECC clones after Cr1+Cr2 and FACS analysis. (I) PCR and Sanger sequencing validation of genotypes on exonuclease-treated DNA, displayed in A, after Cr1+Cr2 for TRE-ECC clone 1 and clone 4. C, negative CRISPR control; NC, non-template control. ( J ) Southern blot, probed with EGFP on HEK293T purified and digested DNA from untreated (C, KpnI ) and Cr1+Cr2 treated cells. X = XbaI , H = HindIII .

Article Snippet: The following vectors were used: lentiCRISPRv2 (a gift from Feng Zhang, Addgene plasmid # 52961) and pUC19 (gift from Joachim Messing, Addgene plasmid # 50005).

Techniques: Fluorescence, CRISPR, Plasmid Preparation, Microscopy, Control, Southern Blot, Activation Assay, Isolation, Clone Assay, Sequencing, Biomarker Discovery, Purification